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Values probably do not represent identical receptor levels. The level of fluorescence is related to the specificity and affinity of the primary antibody, the brightness of the probe number of fluorescein molecules molecule of second antibody ; , the particle or cell size and the number and density of receptors expressed on the cell surface Grogan et al., 1990 ; . Although the values of the peak fluorescence for labelled receptors are similar between granulosa cells and CHO cells, the number of receptors cell was not expected to be the same, particularly in this case where a strong promoter was used to express FSH receptor in CHO cells Kelton et al., 1992 ; . Since the cell size and density of receptor is likely to differ between transfected CHO cells and luteinizing human granulosa cells, direct comparison of receptor levels is probably not valid. Nonetheless, it is valid to compare gonadotrophin receptors labelled with the same antibody on granulosa cells from women with low and normal oestradiol responses to FSH. Although FSH receptor expression was similar on granulosa cells of low and normal responders, there may be alterations in functional capacity of the granulosa cells, or in the ability of receptors to translate hormone binding into intracellular signals. The in-vitro response of human granulosa cells to FSH has been reported to differ between low and normal responders. For example, cultured granulosa cells from low responders secreted less basal oestradiol, but responded to FSH with a greater increase in aromatase activity and oestradiol than higher responders Hurst et al., 1992 ; . Also, basal inhibin B secretion is lower from cultured human granulosa cells of women with low ovarian reserve based on day 3 FSH ; than from cells of women with normal ovarian reserve Seifer et al., 1996 ; . However, since the expression of LH receptor is dependent on appropriate FSH receptor activity, the presence of LH receptor suggests that FSH receptors are functionally intact in low responders.
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Rator AS 104 MTS, Schweinfurt, Germany ; is currently employed. Blood samples from each patient were analyzed for the presence of PBPC daily from day 9 following cyclophosphamide administration until the last collection: leukapheresis was initiated when at least 10 PBPC L were detected. The number of PBPC was determined by direct immunofluorescence on whole blood, using antiCD34 monoclonal antibody and flow cytometry analysis15 with a FACSCalibur analyzer Becton Dickinson, San Jos, CA, USA ; equipped with a filter set for FITC-PE dual-color fluorescence. Data acquisition and analysis were performed with CELL Quest Software and each measurement included at least 40, 000 cells. The frequency of cells expressing CD34 + was calculated as the percentage of all analyzed cells. Dead cells were excluded on the basis of forward- and side-scatter analysis. The number of circulating CD34 + cells per L of blood or the total number of CD34 + in the PBPC collections was obtained by multiplying this percentage by the total number of the leukocytes. A composite collection totalling at least 2106 CD34 + kg was defined as a sufficient yield.
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Sir, I grateful to Dr Shaldon for his comments on the intravenous use of iron dextrin polymaltose ; in France. In fact he is only partly correct in what he says. As I have conrmed with the manufacturing company, Vifor International in Switzerland, iron dextrin was never `ofcially' licensed for i.v. use in France as we dene this term today. It was, however, adopted onto hospital formularies for low-dose administration into the extra-corporeal circuit during haemodialysis and this has now become accepted practice in many units including Dr Shaldon's ; in France. Iron sucrose on the other hand has been granted a Produce Licence for i.v. use by the EMEA regulatory body, and as such it has become widely accepted throughout Europe Dr Jessica Tanner, Medical Director, Vifor International, Switzerland; personal communication ; . I was, however, aware of Dr Shaldon's excellent publications on the subject of low-dose i.v. iron during dialysis and I sorry if my choice of the Sunder-Plassmann and Horl reference has offended him. This article was an Editorial Comment rather than a comprehensive review of the subject and space restraints did not allow every single relevant reference to be included. The aim of my article was to provide a balanced overview of the options for administering i.v. iron without simply recounting my personal opinions, and I felt I had achieved this. Consultant Nephrologist Department of Renal Medicine King's College Hospital London UK Iain C. Macdougall.
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Voices of the Voiceless 229 been unable to go to the scene of the tragedy because they had not been allowed to, came to the hospital. Requisitioned `We have been told that there is a list of the victims posted at the hospital entrance, but there is nothing and there is nobody to inform us, ' protested in a low voice a mother whose son had spent the night at a friend's house in Bentelha. Groups of three to five people, undoubtedly members of the same families, talked in hardly audible voices, as if they were afraid of being overheard. A young soldier stopped a van full of bottled mineral water. He took out two crates without consulting the driver. `They are requisitioned, ' he said before distributing them to people around him. Unearthly Scream Suddenly, there was some sort of a stir in the crowd. People turned to a man who had just got out of a car: He was about 50 years old, brown, small, hard-looking. His clothes were spattered with dry blood on the forearms, chest and knees. He was one of the survivors. A young man, undoubtedly a policeman, held him by the arm. A women in hijab [the Islamic dress], who seemed to know him, rushed on towards him: `Azeddine, hey, Azeddine?' she gasped with difficulty. `May God bless his soul, ' he answered, holding back a sob. The unfortunate woman remained dumb for a moment, then she made a shrieking scream, an unearthly one, filled with an indescribable pain. Other women and men, touched to their hearts' core started weeping. The policemen panicked. Undoubtedly fearing that things might get out of hand, their chief ordered everyone to be moved back from the hospital entrance to the other side of the road. The crowd obeyed. Once again, unarmed civilians have been savagely massacred. And once again the countless questions raised by these countless butcheries will remain unanswered. Who? Why? What is the purpose of this? If, for the authorities, things are clear, in that the responsibility of these horrible bloodbaths is put on Islamic terrorism, on the contrary, for the Algerians, there is a big question mark. Because they cannot understand, they refrain from passing a judgement. `The situation is so complex, ' confessed a journalist of the local press who came to this hospital to get some news in vain, `that all hypotheses are plausible. There is such a large quantity of arms in circulation, so much hatred between the two protagonists and so much misfortunes that anything has now become possible. Only one thing is certain on both sides: the contempt for human life. The doubts of the Algerian public opinion are fed by the Algerian government's persistent refusal to accept the sending [to Algeria] of an international commission of inquiry to shed light on these massacres. When one.
Pollution disaster and Hazardous waste Management only 16% of the revised outlay could be utilized. Grant in aid to DPCC Due to availability of sufficient funds with DPCC, only Rs 25.00 lac were released out of revised outlay of Rs 100.00 lac. Effluent Treatment plants on eighteen major drains which discharge into River Yamuna scheme could not be implemented during 2002-2003 and vesicare.
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Effect of LEV in Combination with L-DOPA. Dyskinesia was significantly reduced following LEV 60 mg kg ; LDOPA combination therapy, but not following LEV 13 mg kg ; L-DOPA combination, during the 0- to 1-, to 2-, and 2to 3-h time periods, compared with L-DOPA alone P 0.05, P 0.01, and P 0.05, respectively; Fig. 1, A, C, and E ; . The.
4 Pathology Candida albicans present in normal oral flora of 20-50% of people Opportunistic infection KOH: hyphae Treatment: Topical or systemic antifungals Maintenance may be needed in immunocompromised patients Associations: Antibiotics, corticosteroids, xerostomia, poor dental hygiene, immunocompromised state References 1. Amagai, M, Tsunoda, K, Zililkens, D, et al. The clinical phenotype of pemphigus is defined by the anti-desmoglein autoantibody profile. J Acad Dermatol. 1999. 40: 167-70. Bolognia, AL, Jorizzo, JL and RP Rapini. Dermatology. 2003. Mosby. 3. Leskaris, G. Color Atlas of Oral Disease 3rd Ed. 2003. Thieme. 4. McKee, PH. Essential Skin Pathology. 1999. Mosby. 5. Nicolas, ME and AN Kalaaji. Immunofluorescence Primer. Mayo Press. 6. Odom, RB, James, WD and TG Berger. Andrew's Diseases of the Skin: Clinical Dermatology. 2000. WB Saunders Company. 7. Sciubba, JJ, Regezi, JA and RS Rogers. PDQ Oral Disease Diagnosis and Treatment. 2002. BC Decker Inc. 8. Scully, C. Handbook of Oral Disease: Diagnosis and Mangagement. 1999. Martin DunitzLtd and vinblastine.
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