MATERIALS AND METHODS Bacterial strains, plasmids, and culture conditions. Bacterial strains and plasmids used in this study are listed in Table 1. E. coli strains were grown at 37C in Luria-Bertani broth or agar. M. smegmatis strains were grown at 37C with shaking 200 rpm; Innova 4300 incubator shaker; New Brunswick Scientific, Edism, N.J. ; in Middlebrook 7H9 broth BBL Microbiology Systems, Cockeysville, Md. ; supplemented with albumin-dextrose complex ADC ; and 0.05% Tween 80 M-ADC-TW broth ; . For analysis of the intracellular amino acid pools, M. smegmatis was grown in a minimal medium based on the formulation of Zygmunt 37 ; and modified as previously described 6 ; . Tryptic soy agar base Difco Laboratories, Detroit, Mich. ; was used for growth of M. smegmatis on solid media. Transformations of E. coli and M. smegmatis were performed by electroporation with a Gene Pulser Bio-Rad Laboratories, Richmond, Calif. ; as described previously 14 ; . The following antibiotics were added to the media when necessary: kanamycin Sigma Chemical Co., St. Louis, Mo. ; at 50 g carbenicillin Sigma ; at 50 g for E. coli strains and kanamycin at 10 g for M. smegmatis strains. Oligonucleotide primers, nucleic acid manipulations, and primer extension analysis. The oligonucleotide primers Integrated DNA Technologies, Inc., Coralville, Iowa ; for PCR amplification of the complete ddl gene from M. smegmatis were SMDDLCF 5 -CGC ATA AGG CCA GGT CAG-3 ; and SMDDLCR 5 -CGA AAA ACC CGT CGT GC-3 ; . The primers for PCR amplification of the ddl gene from M. tuberculosis were DDLATBU 5 -GCT AAG TGC CGA. 12 visits for same condition, same service requires PA for entire episode of care including bi-lateral treatment ; , provided visit limit does not apply. Only initial 12 visits do not require Prior Authorization. Physician Orders include services ordered by the member's PCP and or a specialist, provided benefit allows for self-referral or PCP referred member for evaluation and treatment. PCP Orders should include both visit and length of authorization parameters and should be in writing. If verbal orders are given, a note should be included in the patient's medical chart. Coverage is dependent upon medical necessity, eligibility, contract limitations and exclusions. 2007 BH Benefit Grid FINAL VERSION - 15. University or trade school of their choice. Currently, the Scholarship supports the needs of six students, all former Trinity clients. Some of these students need tuition; others just need help with room and board or transportation. Scholarship funds are made payable directly to landlords, utility companies or colleges to ease the financial stress of these fine young people. In 2006, your contributions have also directly benefited these students: Dale * is in his sophomore year at Dominican University of California. He has received tuition coverage when he needed it most, and he is grateful to all who help him meet his educational goals. Generally, hearings are held for notified applications and are normally open to the public. Hearings may also be held for applications not notified, but then only adversely affected persons are involved. 815 On the whole, public participation is complicated, since more than one resource consent might be needed for the same activity both from territorial authority and the regional council. The other main process including provisions on public participation is in relation to policy statements and plans. After public notification of the proposal anyone may make written submissions, including iwi authorities and hapu, and any one who made a submission has a right to be heard at the hearing. 816 The different planning instruments have slightly different processes, and the participation process for plans and regional policy statements are the most extensive. The form is set out in Schedule 1 of the Act. This process provides for further submissions in response to submissions made by others. In this sense, a debate may develop which contributes to the oral submissions at the hearing.817 During the preparation, the local authorities also have a duty to consult with iwi authorities 818 . Regarding the national environmental standards, the public and iwi authorities must be notified of the proposal and be given time and opportunity to comment on the proposed standard 819 . Generally, the process for participation in relation to the planning instruments takes considerably longer time than the resource consent process, not at least because of the complexity of the planning documents. 820 One prerequisite for effective participation is access to adequate and relevant environmental information. The local authorities have a general duty to gather environment information and to undertake or commission any research necessary to carry out its functions effectively under the Act 821 . The local authorities shall make environmental information available for the public, and the information that must be kept is listed in detail 822 . In relation to hearings, a local authority can withhold sensitive information to avoid serious offence to Maori customs and rights, to avoid the disclosure of the location of sacred places, or to avoid disclosure of trade secrets 823 . Otherwise, private or commercial details may be withheld from the public based on criteria in the Privacy Act 1993 and Official Information Act 1982.